Étude comparée des gels hydroalcooliques importés et ceux produits au Bénin, vendus dans les supermarchés de la ville de Cotonou / NA

Depuis le début de la crise épidémique liée au virus Covid-19, pour de nombreux citoyens, se laver les mains est devenu un rituel nécessaire. Le gel hydroalcoolique constitue une solution alternative qui a été popularisée par les recommandations des autorités sanitaires dans le cas d'indisponibilité du lavage des mains. Que cela soit en pharmacie, en supermarché, ou sur internet, de nombreux sites de commerce proposent ces produits à la vente. Toutefois l'appellation ne garantit pas l'efficacité de chaque produit vendu. C'est ainsi que la présente étude s'est attelée à l'évaluation de la qualité des gels hydroalcooliques (GHA) utilisés pour la désinfection des mains au cours de la crise sanitaire liée au Coronavirus. Au total, 28 échantillons dont 23 importés et 5 locaux ont été collectés dans 16 supermarchés de la ville de Cotonou. Ils ont été soumis au test d'inspection visuelle, aux tests organoleptiques ainsi qu'aux analyses physico-chimiques et microbiologiques. Au terme de l'étude, 17,86% des GHA ont été déclarés non-conformes par rapport aux inspections visuelles, 39,28% par rapport aux tests organoleptiques, 21% par rapport au pH et à la densité et enfin 21% de non-conformité aux tests microbiologiques. Le taux de non-conformité est plus important dans le camp des gels importés ce qui atteste l'effort des producteurs locaux pour le respect des normes promulguées par les autorités sanitaires

Since the start of the epidemic crisis linked to the Covid-19 virus, for many citizens, washing their hands has become a necessary ritual. Hydroalcoholic gel constitutes an alternative solution which has been popularized by the recommendations of the health authorities in the case of hand washing unavailable. Whether in pharmacies, supermarkets, or on the internet, many shopping sites offered these products for sale. However, this name does not guarantee the effectiveness of each product sold. The present study has been set out to assess the quality of hydroalcoholic gels (GHA) used for hand disinfection during the health crisis linked to the Coronavirus. In total, 28 samples, of which 23 were imported and 5 locals, were collected in 16 supermarkets in the city of Cotonou. They were subjected to visual inspection test, organoleptic tests as well as physico-chemical and microbiological analyzes. At the end of the study, 17.86% of GHAs were declared non-compliant with respect to visual inspections, 39.28% with respect to organoleptic tests, 21% with respect to pH and density and finally 21% of noncompliance with microbiological testing. The rate of non-compliance is higher in the imported gel camp, which attests to the efforts of local producers to comply with the standards promulgated by the health authorities

Cymbopogon giganteus Chiov. essential oil: Direct effects or activity in combination with antibiotics against multi-drug resistant bacteria

The discovery of new antimicrobial agents is necessary due to the emergence of multi-drug bacterialresistance. The aim of this work was to study the direct and indirect antimicrobial activity of a Beninesesample of Cymbopogon giganteus essential oil (EOCG) on multi-drug resistant clinical bacteria, its chemicalcomposition, and its cytotoxicity. Direct antimicrobial activity was tested by determination of minimalinhibitory concentration (MIC), and indirect activity, by determining Fractional Inhibitory ConcentrationIndex using checkerboard [fractional inhibitory concentration indices (FICI); synergy: FICI ≤ 0.5; additivity:0.5 < FICI ≤ 1]. EOCG composition was determined by GC-MS and GC-FID and cytotoxicity was determinedby 3-(4,5-dimethylthiazol-2-yl)-2,5-diphényltetrazolium bromide (MTT) assay. p-Menthane derivatives(54.87%) and limonene (12.07%) were detected as major compounds by GC analysis. Our results confirmedthe direct antimicrobial activity of EOCG, but here on clinical resistant strains (MIC from 0.125% v/v to 0.5%v/v). We also show synergistic effects between EOCG and amoxicillin with FICI ranges of 0.12–0.5 againsttwo Escherichia coli resistant clinical strains, synergistic to additive effects between EOCG and colistin oroxacillin/ampicillin, respectively, against Pseudomonas aeruginosa PA544 and Staphylococcus epidermidisSECN361 (two resistant clinical isolates). Our results also indicate that EOCG had low cytotoxicity(IC50: 67.06 ± 2.694 μg/ml).